Next Generation Nucleic Acid Extraction technology NGEx®
Oncodia introduces a fully automated next generation technology for nucleic acid extraction from formalin-fixed, paraffin-embedded (FFPE) tissue sections using paramagnetic beads. The Oncodia’s NGEx® technology enables serial extraction of DNA and RNA from the same biospecimen where the isolated nucleic acids are of optimal quality for next generation sequencing and other downstream applications.
The NGEx technology
Oncodia develops processes and know-how for nucleic acid extraction from human tissue specimens based on the Next Generation Extraction technology (NGEx®). The technology has been available since 2012 and ever since delivers an innovative new method for automated nucleic acid extractions both for clinical and research use.
Oncodia NGEx features
- The only truly fully automated extraction solution.
- No manual reagent handling.
- Sequential extraction of both DNA and RNA in a single workflow from the same tissue specimen.
- IVDR compliant for in vitro diagnostics.
- Xylene-free deparaffinization.
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For more information on licensing and use of our products, please contact us.
The Oncodia NGEx® nucleic acid isolation protocols use intuitive software, novel lysis buffer solutions and magnetic separation of DNA and RNA-bound bead particles enabling standalone DNA/total RNA or proprietary serial extraction of both DNA and RNA from the same tissue sample. The NGEx purification kits consist of plastic-ware, reagents packed into prefilled cartridges and a deparaffinisation reagent.
Software and Extraction Robots
The NGEx® purification technology utilizes a convenient bench-top Magtration® System magLEAD 12GC and 12GC Plus/UV robots (Precision System Science Co.,Ltd.) with the ability to simultaneously process up to 12 samples (Magtration® system).The Magtration® Technology platform enables separation and re-suspension of magnetic beads within a disposable tip, reducing the risk for cross contamination between samples.
CAT # ES-K110FP-C
The FFPE DNA Purification Kit is optimized for rapid and efficient purification of genomic DNA from FFPE tissue samples. Purified DNA is of high yield and quality and is suitable for use in next generation sequencing, real time RT-PCR, PCR and other downstream application. The protocol requires a total run time of 3 hours 40 mins.
CAT # ES-K210FP-C
The FFPE Total RNA Purification Kit allows a rapid and efficient extraction of total RNA from FFPE tissue samples. Tissue deparaffinisation is safely and effectively achieved without the use of organic solvents. Purified RNA is of optimal quality for next generation sequencing, real time RT-PCR, PCR and other downstream applications. The protocol requires a total run time of 2 hours 15 mins.
CAT # ES-K110210FP-C
The FFPE DNA/RNA Purification Kit is optimized to allow the sequential purification of genomic DNA and total RNA from the same FFPE tissue sample. DNA and total RNA are of suitable quality for next generation sequencing, real time RT-PCR, PCR and other downstream application, with a total run time of 3 hours 10 min (short lysis) or 4 hours 30 mins (long lysis).
CAT # ES-K110F
This kit is optimized to allow the purification of genomic DNA from fresh-frozen tissue samples embedded in OCT. The kit contains prefilled reagents cartridges and consumables for the fully automated extraction process. DNA is of suitable integrity for next generation sequencing, real time PCR, PCR and other downstream applications. This protocol is designed to minimize hands-on time and has a total run time of 3 hours 20 mins.
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