Complete solutions for nucleic acid extraction in molecular pathology
Next Generation Nucleic Acid Extraction technology NGEx®
Oncodia introduces a fully automated next generation technology for nucleic acid extraction from formalin-fixed, paraffin-embedded (FFPE) tissue sections using paramagnetic beads. The Oncodia’s NGEx® technology enables serial extraction of DNA and RNA from the same biospecimen where the isolated nucleic acids are of optimal quality for next generation sequencing and other downstream applications.
The NGEx technology
Oncodia develops processes and know-how for nucleic acid extraction from human tissue specimens based on the Next Generation Extraction technology (NGEx®). The technology has been available since 2012 and ever since delivers an innovative new method for automated nucleic acid extractions both for clinical and research use.
Oncodia NGEx features
- The only TRULY fully automated extraction solution.
- No manual reagent handling.
- Sequential extraction of both DNA and RNA in a single workflow from the same tissue specimen.
- CE marked for IVD.
- Xylene-free deparaffinization.
The Oncodia NGEx® nucleic acid isolation protocols use intuitive software, novel lysis buffer solutions and magnetic separation of DNA and RNA-bound bead particles enabling standalone DNA/total RNA or proprietary serial extraction of both DNA and RNA from the same tissue sample. The NGEx purification kits consist of plastic-ware, reagents packed into prefilled cartridges and a deparaffinisation reagent.
Software and Extraction Robots
The NGEx® purification technology utilizes a convenient bench-top Magtration® System magLEAD 12GC and 12GC Plus/UV robots (Precision System Science Co.,Ltd.) with the ability to simultaneously process up to 12 samples (Magtration® system).The Magtration® Technology platform enables separation and re-suspension of magnetic beads within a disposable tip, reducing the risk for cross contamination between samples.
Extracting DNA from FFPE Tissue Biospecimens Using User-Friendly Automated Technology: Is There an Impact on Yield or Quality?
Mathieson, W. et al. Biopreservation and Biobanking, 2018 May 3. doi: 10.1089/bio.2018.0009
Viruses in case series of tumors: Consistent presence in different cancers in the same subjectArroyo Mühr, L. S. et al. PLOS ONE 12, e0172308 (2017)
Efficient and scalable serial extraction of DNA and RNA from frozen tissue samplesMathot L, Lindman M, Sjöblom T. Chem Commun 2011;47:547-9.
Automated serial extraction of DNA and RNA from biobanked tissue specimens
Mathot L, Wallin M, Sjöblom T. BMC Biotechnol, 13, 66.
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