NGEx products

Extraction

Next Generation Nucleic Acid Extraction technology NGEx®

Oncodia introduces a fully automated next generation technology for nucleic acid extraction from formalin-fixed, paraffin-embedded (FFPE) tissue sections using paramagnetic beads. The Oncodia’s NGEx® technology enables serial extraction of DNA and RNA from the same biospecimen where the isolated nucleic acids are of optimal quality for next generation sequencing and other downstream applications.

The NGEx technology

Oncodia develops processes and know-how for nucleic acid extraction from human tissue specimens based on the Next Generation Extraction technology (NGEx®). The technology has been available since 2012 and ever since delivers an innovative new method for automated nucleic acid extractions both for clinical and research use.

Quality Assurance

ExScale has implemented a quality management system according to ISO13485 with CE marked products according to Directive 98/79/EC.

Oncodia NGEx features

  • The only TRULY fully automated extraction solution.
  • No manual reagent handling.
  • Sequential extraction of both DNA and RNA in a single workflow from the same tissue specimen.
  • CE marked for IVD.
  • Xylene-free deparaffinization.

Purification kits

The Oncodia NGEx® nucleic acid isolation protocols use intuitive software, novel lysis buffer solutions and magnetic separation of DNA and RNA-bound bead particles enabling standalone DNA/total RNA or proprietary serial extraction of both DNA and RNA from the same tissue sample. The NGEx purification kits consist of plastic-ware, reagents packed into prefilled cartridges and a deparaffinisation reagent.

Software and Extraction Robots

The NGEx® purification technology utilizes a convenient bench-top Magtration® System magLEAD 12GC and 12GC Plus/UV robots (Precision System Science Co.,Ltd.) with the ability to simultaneously process up to 12 samples (Magtration® system).The Magtration® Technology platform enables separation and re-suspension of magnetic beads within a disposable tip, reducing the risk for cross contamination between samples.

Product portfolio

Products

CAT # ES-K110FP-C
The FFPE DNA Purification Kit is optimized for rapid and efficient purification of genomic DNA from FFPE tissue samples. Purified DNA is of high yield and quality and is suitable for use in next generation sequencing, real time RT-PCR, PCR and other downstream application. The protocol requires a total run time of 3 hours 40 mins.

FFPE DNA Purification Kit 48 reactions on Magtration® System

Cat # ES-K110FP-C

The FFPE DNA Purification Kit is optimized for rapid and efficient purification of genomic DNA from FFPE tissue samples. Purified DNA is of high yield and quality and is suitable for use in next generation sequencing, real time RT-PCR, PCR and other downstream application. The protocol requires a total run time of 3 hours 40 mins.

FFPE Total RNA Purification Kit 48 reactions on Magtration® System

Cat # ES-K210FP

The FFPE Total RNA Purification Kit allows a rapid and efficient extraction of total RNA from FFPE tissue samples. Tissue deparaffinisation is safely and effectively achieved without the use of organic solvents. Purified RNA is of optimal quality for next generation sequencing, real time RT-PCR, PCR and other downstream applications. The protocol requires a total run time of 2 hours 15 mins.

FFPE DNA/RNA Purification Kit 48 reactions on Magtration® System

Cat # ES-K110210FP-C

The FFPE DNA/RNA Purification Kit is optimized to allow the sequential purification of genomic DNA and total RNA from the same FFPE tissue sample. DNA and total RNA are of suitable quality for next generation sequencing, real time RT-PCR, PCR and other downstream application, with a total run time of 3 hours 10 min (short lysis) or 4 hours 30 mins (long lysis).

Fresh-frozen DNA/RNA Purification Kit 48 reactions on Magtration® System

Cat # ES-K110210F

This kit is optimized to allow the sequential purification of genomic DNA and total RNA from the same fresh-frozen tissue sample embedded in OCT or up to 1 million cells. The kit contains prefilled reagents cartridges and consumables for the fully automated extraction process. DNA and total RNA are of suitable quality for next generation sequencing, real time RT PCR, PCR and other downstream application. This protocol is designed to minimize hands-on time and has a total run time of 2 hours 15 mins.

Fresh-frozen DNA Purification Kit 48 reactions on Magtration® System

Cat # ES-K110F

This kit is optimized to allow the purification of genomic DNA from fresh-frozen tissue samples embedded in OCT. The kit contains prefilled reagents cartridges and consumables for the fully automated extraction process. DNA is of suitable integrity for next generation sequencing, real time PCR, PCR and other downstream applications. This protocol is designed to minimize hands-on time and has a total run time of 3 hours 20 mins.

Resources

References

Extracting DNA from FFPE Tissue Biospecimens Using User-Friendly Automated Technology: Is There an Impact on Yield or Quality?

Mathieson, W. et al. Biopreservation and Biobanking, 2018 May 3. doi: 10.1089/bio.2018.0009

 

Viruses in case series of tumors: Consistent presence in different cancers in the same subject

Arroyo Mühr, L. S. et al. PLOS ONE 12, e0172308 (2017)
 

Efficient and scalable serial extraction of DNA and RNA from frozen tissue samples

Mathot L, Lindman M, Sjöblom T. Chem Commun 2011;47:547-9.
 

Automated serial extraction of DNA and RNA from biobanked tissue specimens

Mathot L, Wallin M, Sjöblom T. BMC Biotechnol, 13, 66.

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